Grasas y Aceites, Vol 50, No 3 (1999)

Synthesis of structured triacylglycerols containing medium-chain and long-chain fatty acids by interesterification with a stereoespecific lipase from Mucor miehei.


https://doi.org/10.3989/gya.1999.v50.i3.656

Susana Nieto
Laboratorio de Lípidos y Antioxidantes, INTA, Universidad de Chile., Chile

Julio Sanhueza
Laboratorio de Lípidos y Antioxidantes, INTA, Universidad de Chile., Chile

Alfonso Valenzuela
Laboratorio de Lípidos y Antioxidantes, INTA, Universidad de Chile., Chile

Abstract


The preparation of structured triacylglycerols sn-1, sn-3 dilauryl, sn-2 eicosapentaenoyl glycerol and sn-1, sn-3 dilauryl, sn-2 docosahexaenoyl glycerol by enzymatic interesterification under restricted water availability is described. Laurie acid, one of the substrates for interesterification, was obtained by the controlled hydrolysis of coconut oil by a non-specific lipase obtained from Candida cylindracea. The fatty acid was separated from the hydrolysis products by silverresin column chromatography and converted to methyl ester, sn-2 Eicosapentaenoyl glycerol and sn-2 docosahexaenoyl glycerol were prepared by the hydrolysis of fish oil by the sn-1, sn-3 stereospecific immobilized lipase Lipozyme IM-20 obtained from Mucor miehei as described in the accompanying paper. The interesterification was carried out in a water jacketed glass reactor and the triacylglycerol products were separated and recovered through aluminum oxide column chromatography The interesterification procedure described allows to obtain In laboratory scale structured triacylglycerols containing medium-chain fatty acids at the sn-1 and sn-3 positions and long-chain polyunsaturated fatty acid from marine origin at the sn-2 glycerol position.

Keywords


Interesterification; Lipase (Mucor miehei); Triacylglycerol

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