Grasas y Aceites, Vol 65, No 2 (2014)

Characterization of apple seed oil with Denomination of Origin from Asturias, Spain

J. C. Bada, M. León-Camacho, P. Copovi, L. Alonso

DOI: http://dx.doi.org/10.3989/gya.109813

Abstract


The content and composition of lipids isolated from the seeds of seven apple species from Asturias (Spain) were characterized. The highest content in oil corresponded to Collao (22.73±0.81 g·100 g-1 of seed), followed by Raxao and Riega (20.19±0.74 g·100 g-1 of seed; 19.67±0.85 g·100 g-1 of seed), respectively. The linoleic acid was found to be the main component in Limón Montés (60.78±3.07%) followed by Riega (60.01±3.41%). Solarina seed oil was the one with the highest content in total sterols (558.52±9.42 mg·100 g-1 of oil) while Blanquina was the one that presents the lowest amount (166.55±1.89 mg·100 g-1 of oil). Phosphatidylcholine (70.58±3.85%) was found to be the main constituent in Blanquina followed by Collaos (55.55±2.96%). Raxao presented the highest content in β-tocopherol (125.29±12.62) and α-tocopherol was the most important tocopherol in Limón Montés (84.68±5.61 mg·kg-1 of oil). The main triglycerides were LLP (41.17±1.98-39.32±1.66%) followed by LLL (27.12±1.32−17.80±1.96%). Good separation among specie samples according to the statistical analysis of the principal component (PCs) and linear discriminant analysis (LDA) was obtained.

Keywords


Apple; Fatty acids; Oils; Phospholipids; Sterols; Tocopherols; Triglycerides

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References


Alonso L. 1993. Fat modification in Afuega´ l Pitu cheese during ripening by gas capillary gas chromatography. J. Am. Oil Chem. Soc. 70, 1035−1037. http://dx.doi.org/10.1007/BF02543033

Anwar F, Latif S, Ashraf M. 2006. Analytical characterization of hemp (cannabis sativa) seed oil from different agroecological zones of Pakistan. J. Am. Oil Chem. Soc. 83, 323−329. http://dx.doi.org/10.1007/s11746-006-1207-x

BOE (Spanish Oficial Bolletin). Cicer origen denomination from Asturias. n° 38. 5524–5533. 11/2/2003.

Cai YQ, Keiko A, Terutaka K, Hidetoyo T, Minoru K. 2003. The relationship between diet and mortality of cancer and non-communicable disease in Japan. J. Nanjing Med. University 17, 95−111.

Coomans D, Massart, DL, Kaufman L, 1979. Anal. Chim. Acta 112, 97–122. http://dx.doi.org/10.1016/S0003-2670(01)83513-3

Europen Union, Official Diary, reglament n°. 479/2008.

Gardine WP. 1997. Statistical analysis methods for chemistry, Royal Society of Chemistry. Cambridge, pp. 313–328.

Hu F, 2006. Study on the international competitiveness of apple in China. Productivity Res. 10, 17−19.

International Union of Pure and Applied Chemistry (IUPAC). 1987a. Standard Methods for the Analysis of Oils, Fats and Derivatives: Method 1122. Determination of oil content (extraction method). 7ªedn. Eds. C. Paquot, A. Haufenne, Blackwell Scientific Publications, Oxford, UK, 1987d, pp. 14−16.

International Union of Pure and Applied Chemistry (IUPAC). 1987b. Standard Methods for the Analysis of Oils, Fats and Derivatives: Method 1121. Determination of moisture and volatiles matter content. 7ªedn. Eds. C. Paquot, A. Haufenne. Blackwell Scientific Publications, Oxford, UK, pp. 13−16.

International Union of Pure and Applied Chemistry (IUPAC). 1987c. Standards Methods for the Analysis of Oils, Fats and Derivatives. Methods 2301. Preparation of the fatty acid methyl esters. 7ªedn. Eds. C. Paquot, A. Haufenne. Blacwell Scientific Publications, Oxford, UK, pp. 123−129

International Union of Pure and Applied Chemistry (IUPAC). 1987d. Standard Methods for the Analysis of Oils, Fats and Derivatives: Method 2411. Identification and determination of tocopherols. 7ªedn. Eds. C. Paquot, A. Haufenne, Blackwell Scientific Publications, Oxford, UK, pp. 174−219.

Jolliffe I T. 2002. Principal Components Analysis, Second ed., Springer, New York.

Kris-Etherton PM, Etherton TD. 2003. The impact of the changing fatty acid profile on fats on the diet assessment and health. J. Food Comp. Anal. 16, 373−378. http://dx.doi.org/10.1016/S0889-1575(03)00052-8

Lei Tian H, Zhan P, Li KX. 2010. Analysis of components and study on antioxidant and antimicrobial activities of oil in apple seeds. Int. J. Food Sci. Nutr. 61, 395−403. http://dx.doi.org/10.3109/09637480903535772 PMid:20128637

León-Camacho M, Morales MT. 2000. Gas and liquid chromatography: Methodology applied to olive oil. In: Handbook of olive oil: analysis and properties, Eds. J. L. Harwood, R. Aparicio, Aspen, Gaithersburg, MA (USA), pp. 159–208.

Liang YL, Dai CY, Li B, Shu KX. 2006. Study on extracting technology of total flavone in mustard leaf. Food Res. Develop. 27, 73−75.

Maazouzi C, Masson G, Izquierdo, MS, Pihan JC. 2007. Fatty acid composition of the amphipod Dikerogammarus villous: feedind strategies and trophic links. Comparativee Biochemistry and Physiology-Part A: Molecular & Integrative physiology, 147, 868−875.

Ohama H, Ikeda H, Moriyama H. 2006. Health foods and foods with health claims in Japan. Toxicology. 17, 73−75.

Official Journal of the Commission of the European Communities. 1991. Regulation N° 2568/91, L248, September 9.

StatSoft Inc. 1995. Statistica 5.5 for Windows Computer Program Manual, Tulsa, OK. StatSoft, Inc. 1995. 2300 East 14th Street, Tulsa, OK 74104.

Stone NJ, Kushner R. 2000. Effects of dietary modification and treatment of obesity: Emphasis on improving vascular outcomes. Med. Clin. N. Am. 84, 95−122. http://dx.doi.org/10.1016/S0025-7125(05)70209-1

Yu X, Van de Voort FR, Li Z, Yue T. 2007. Proximate composition of the aple seed and characterization of its oil. Int. J. Food Eng. 3, 1−8. http://dx.doi.org/10.2202/1556-3758.1283

Yukui R, Wenya W, Rashid F, Qing L. 2009. Fatty acids composition of apple and pear seed oils. Int. J. Food Prop. 12, 774−779. http://dx.doi.org/10.1080/10942910802054320

Zlatanov M, Ivanov S, Antonova G, Kouleva I. 1997. Study of phosphopholipids composition of Rosaceae seed Oil. Rev. Ital. Sost. Grasse, 74, 409−410.




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