Application of high-temperature gas chromatography to the analysis of used frying fats
DOI:
https://doi.org/10.3989/gya.109409Keywords:
Dimeric FAME, High-temperature gas chromatography, Oxidized monomeric FAME, Polar compounds, Used frying oilsAbstract
The determination of polar compounds is the most commonly applied technique in the analysis of used frying fats and oils. High-temperature gas chromatography allows for a quantitative determination of oxidized monomeric FAME and dimeric FAME thus giving extra information on oil degradation starting from the fraction of polar compounds. Polar compounds are transesterified and methyl esters are separated in a VF-5ht Ultimetal column (150 °C -held for 5 min- rising at 5 °C min-1 to 370 °C and held for 5 min) using methyl tricosanoate as internal standard. Results are compared with those obtained by more complex alternative methodology using high-performance size-exclusion chromatography.
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Barrera-Arellano D, Márquez-Ruiz G, Dobarganes MC. 1997. A simple procedure to evaluate the performance of fats and oils at frying temperatures Grasas y Aceites 48, 231-235.
Berdeaux O, Márquez-Ruiz G and Dobarganes MC. 1999. Selection of methylation procedures for quantitation of short-chain glycerol-bound compounds formed during thermoxidation. J Chromatog A 863, 171-181. doi:10.1016/S0021-9673(99)00974-7
Berdeaux O, Dutta PC, Dobarganes MC, Sébédio JL. 2009. Analytical methods for quantification of modified fatty acids and sterols formed as a result of processing. Food Anal Methods 2, 30-40. doi:10.1007/s12161-008-9055-y
Choe E, Min, DB. 2007 Chemistry of de ep-fat frying oils: J Food Sci. 72, R77-R86. doi:10.1111/j.1750-3841.2007.00352.x PMid:17995742
Dobarganes MC, Márquez Ruiz G. 1998. Regulation of used frying fats and validity of quick tests for discarding the fats. Grasas Aceites, 49, 331-335.
Dobarganes MC, Pérez-Camino MC, Gutiérrez González- Quijano R. 1984. Métodos analíticos de aplicación en grasas calentadas. I. Determinación de ésteres metílicos no alterados. Grasas y Aceites 35, 172-177.
Dobarganes MC, Velasco J, Dieffenbacher A. 2000. The determination of polar compounds, polymerised triacylglycerols, oxidized triacylglycerols and diacylglycerols in fats and oils. Pure Appl. Chem. 72, 1563-1575. doi:10.1351/pac200072081563
European Standard EN14105. 2003. Fat and oil derivatives - Fatty Acid Methyl Esters (FAME) - Determination of free and total glycerol and mono-, di-, triglyceride contents. European Committee for Standardization, Brussels, Belgium.
IUPAC. 1987. Standard methods for the analysis of oils, fats and derivatives. Standard Method 2.507-International Union of Pure and Applied Chemistry. Pergamon Press, Oxford.
Marmesat S, Velasco J, Dobarganes MC. 2008. Quantitative determination of epoxyacids, ketoacids and hydroxyacids formed in fats and oils at frying temperatures. J Chromatog. A 1211, 129-134.
Márquez Ruiz G, Pérez-Camino MC, Dobarganes MC. 1990. Combination of adsorption and size-exclusion chromatography for the determination of fatty acid monomers, dimers and polymers. J. Chromatog. A 514, 37-44. doi:10.1016/S0021-9673(01)89374-2
Navas JA, Tres A, Codony R, Guardiola F. 2007. Optimización de métodos analíticos para la evaluación de la calidad de grasas y aceites utilizados en el proceso de fritura en continuo. Grasas y Aceites 58, 148-153.
Orthoefer FT, List GR. 2007. Evaluation of used frying oil. In Deep frying: chemistry, nutrition and practical applications (2nd edition), ed. M.D. Erickson, 329-342. Champaign, IL: AOCS Press.
Ruiz Méndez MV, Marmesat S, Liotta A, Dobarganes MC. 2008a. Analysis of used frying fats for biodiesel production. Grasas y Aceites 59, 45-50.
Ruiz-Méndez MV, Liotta A, Marmesat S, Dobarganes MC. 2008b. Detection of the presence of used frying oil as raw material in biodiesel production. Grasas y Aceites 59, 383-388. doi:10.3989/gya.2008.v59.i1.491
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